Examination of endocrine disruptor effects in neuroendocrine systems, in vivo and in vitro

The effects of environmental loads (physical, chemical, biological) interfere with human homeostatic psycho-neuroendocrine-immune mechanisms. Clarifying the role of the triggered effects and their impact factors became an acute task for the 21st century. The aim of the present study was to investigate the effects of chemical (aromatic/halogenated hydrocarbons) and physical (extreme low frequency and dose electromagnetic fields: ELF-EMF) environmental loads as endocrine disruptors (ED). In addition, we have investigated the role of these factors in cell and individual exposure to clarify the mechanisms induced. We considered it particularly important to study the relationship between indirect and direct effects in cell transformation events associated with endocrine regulatory disorders. In our work, we developed in vivo (Wistar rat chlorobenzene treatment through gastric tube; ♀ Wistar rats treatment by subcutaneous estrogen implantation; treatment of turkey with ELF-EMF), and in vitro (neurohypophysis, adenohypophysis monolayer cell culture) exposure models and standardized them for general viability and/or specific functional attractors (mechanism cycles). The chemical agents tested were hexachlorobenzene: 1,2,4-trichlorobenzene = 1:1 (mClB); 1,4-dichlorobenzene (dClB); phenuron (PU), monuron (MU) and diuron (DU) as ED investigated for dose and time dependence. It has been found that said agents have ED effects on OT, AVP, ACTH and PRL release. Furthermore, we have determined the human toxicity potential(HTP) values for dClB in the chemical exposures studied. We have demonstrated that ELF-EMF is a cellular ED which modifies the functions of cell membrane receptors (G proteins) involved in regulatory mechanisms. We developed an adenohypophyseal prolactinoma (PRLOMA) model by estrogen stimulation through a positive feed-back mechanism. We have experimentally demonstrated that the functional derangement of the PRLOMA-like adenohypophysis with ED agents often triggers a non-compensable event cascade when compared to normal cell function. Based on our in vitro results, we also investigated the role of ED effects in the background of thyroid cell transformation disorders diagnosed in medical practice. We found a correlation between ED exposures and anti-TG or anti-TPO-labeled thyroid malignant tumors, respectively. Our findings reveal that mClB, PU, MU, DU, and ELF-EMF can be regarded as ED. In addition, we have demonstrated that chronic endocrine regulatory disorders may induce cell transformation so that the target cells of which show different behavior in their regulation compared to healthy cells. We have found a relationship between the real thyroid cell transformations (malignant tumors), the presence of anti-TG/anti-TPO markers and ED in the medical histories of the patients

Sugar substitute compounds as environmental exposures - aspects from neuroendocrine functions

It is an extremely interesting question to what extent elements of psychic activity (e.g. cognition) in social existence can change by mediating neuroendocrine communication when it is necessarily altered by chemical environmental influences (e.g. nutritional biological agents) through real technosphere exposures. In the processes of learning and memory, biological mechanisms regulated by the neuro-endocrine system appear predominantly, showing a network relationship with essential local system properties. Exposures (dietary supplements) are tested on in vitro models by monitoring the events of neuro-endocrinological communication (hormone secretions, monoamine /adrenaline and serotonine/) and their changes in exposure

Magnesium-dependent atpase as a possibility for the investigation of deponating environmental loads in a hepatocyte model

Chemicals that load the environment can be chlorobenzenes with massive chemical stability. They induce a dose-dependent toxic effect in the cells of affected tissues, and because of their high frequency of occurrence in the food chain, they can be used as expositors in environmental exposure models. In this work, we wanted to investigate the magnesium-dependent ATPase activity of chlorobenzenes in hepatocytes

Chlorobenzene compounds as possible immuno-disruptor agents

Dichlorobenzenes are lipophilic, depositable, colorless liquids that appear as an exposure factor because they are continuously present in households, but are also used in agriculture in large quantities in e.g. insecticides and fungicides. As there is a constant interaction between the living systems and its environment and the internal organizational stability of biological systems is controlled by homeostasis, these agents may disrupt the homeostasis, therefore it is especially important to study the effect of these compounds on the immune system

Effect of subtoxic dose, extreme low frequency (ELF) electromagnetic field (EMF) treatments on liver enzyme changes in in vivo Turkey model experiments

Natural electromagnetic field background radiation in Earth is 20-30 µT, which is a condition in terrestrial evolution. Today, however, in the technosphere-determined environment, intermittent extreme low-frequency electromagnetic fields are predominant. In the present work, we aim to investigate this topic area by in vivo systematic study of subtoxic and chronic electromagnetic field exposures in a turkey model

A fibrocyte model for monitoring environmental chemicals

Human activity affects all elements of the Earth's environment and the system of relationships between them. Chlorobenzenes created during chemicalization are capable of modulating the adaptation potential of biological organisms and because of their high frequency of occurrence in the food chain, they can be used as expositors in environmental exposure models. It is necessary to develop a biological model system suitable for the investigation of environmental pollutant chemical agents, which indicates changes quickly and easily

Environmental effect on thyroid disfunction

The challenges of endocrinology, including those of endocrine disruption, force today’s medical science to face the numerous environmental health risks. Disruption of the endocrine system, which in reality affects the unity of the psycho-neuroendocrine immune system, may play a role in the development of many diseases. In this work, one of the basic questions was whether the environmental loads can cause disease (transformation disorders and processes) in the thyroid gland. Our aim was to develop the novel diagnostic method or environmentrelated thyroid diseases. The endocrine disrupting compounds play an important role in inflammation and transformation of the thyroid gland. For this reason, upgrading any diagnostic method by adding environmental parameters is advised